pDNR-hSox2
(Plasmid
#49389)
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Purposedonor vector for cloning human Sox2 with 6xHN tag
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 49389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDNR-dual
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4900
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Vector typeCre/Lox ; donor vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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Alt namehuman Sry
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SpeciesH. sapiens (human)
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Insert Size (bp)953
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MutationTGA (stop) to CCA (Pro) at nt 1027 with respect to GenBank ID BC013923
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GenBank IDBC013923
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
- Promoter none
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Tag
/ Fusion Protein
- 6xHN (6 repeating His-Asn subunits) (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer CAT-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
from ATCC #10658153
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDNR-hSox2 was a gift from Randall McKinnon (Addgene plasmid # 49389 ; http://n2t.net/addgene:49389 ; RRID:Addgene_49389)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/88/24/73dd287a-423a-11e3-943a-000c29055998.png.940x940_q85_autocrop.png)